• Product NameRecombinant Human Interleukin-4(rHu IL-4)
• Brief DescriptionRecombinant Protein
• Host SpeciesE.coli
• Purification> 98 % by SDS-PAGE and HPLC analyses.
• Species ReactivityHu
• Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.2 ng ml, corresponding to a specific activity of > 5.0 ⅹ 106 IU mg.
• EndotoxinLess than 1.0 EU/μg of rHuIL-4 as determined by LAL method.
• Target NamerHu IL-4
• Alternative Names    BSF-1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra.
• Accession No. accession:P05112
  GeneID:3565
• UniprotP05112
• Gene ID3565;
• Calculated MW Approximately 15.0 kDa, a sing
• SDS-PAGE MW Sterile Filtered White lyophil
• Target Sequence HKCDITLQEI IKTLNSLTEQ KTLCTELTVT DIFAASKNTT EKETFCRAAT VLRQFYSHHE KDTRCLGATA QQFHRHKQLI RFLKRLDRNL WGLAGLNSCP VKEANQSTLE NFLERLKTIM REKYSKCSS
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg mL. Stock solutions should be apportio
• Storage This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.

Interleukin-4 (IL-4) is a pleiotropic cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells and produced by by mast cells, activated T cells and bone marrow stromal cells. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. In addition, It enhances both secretion and cell surface expression of IgE and IgG1 and also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. The human IL-4 has a compact, globular fold, stabilised by 3 disulphide bonds. The human IL-4 shares about 40% aa sequence identity with mouse rat IL-4 and they are species-specific in their activities.

1. Sokol CL, Barton GM, Farr AG, et al. 2008. Nat Immunol. 9:310-8.1. Sokol CL, Barton GM, Farr AG, et al. 2008. Nat Immunol. 9:310-8. 2. Hershey GK, Friedrich MF, Esswein LA, et al. 1997. N Engl J Med. 337:1720-5. 3. Yokota T, Otsuka T, Mosmann T, et al. 1986. Proc Natl Acad Sci U S A. 83:5894-8. 4. Eder A, Krafft-Czepa H, Krammer PH. 1988. Nucleic Acids Res. 16:772. 5. Walter MR, Cook WJ, Zhao BG, et al. 1992. J Biol Chem. 267:20371-6.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting