• Product NameRecombinant Human Interleukin-6(rHu IL-6)
• Brief DescriptionRecombinant Protein
• Host SpeciesE.coli
• Purification> 96 % by SDS-PAGE and HPLC analyses.
• Species ReactivityHu
• Biological ActivityAssay #1: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using IL-6-dependent murine 7TD1 cells is less than 0.1 ng ml, corresponding to a specific activity of > 1.0 ⅹ 107 IU mg. Assay #2: Fu
• EndotoxinLess than 1.0 EU/μg of rHuIL-6 as determined by LAL method.
• Target NamerHu IL-6
• Alternative Names    BSF-2, CDF, Hybridoma growth factor, IFN-beta-2.
• Accession No. accession:P05231
  GeneID:3569
• UniprotP05231
• Gene ID3569;
• Calculated MW Approximately 20.7 kDa, a sing
• SDS-PAGE MW Sterile Filtered White lyophil
• Target Sequence VPPGEDSKDV AAPHRQPLTS SERIDKQIRY ILDGISALRK ETCNKSNMCE SSKEALAENN LNLPKMAEKD GCFQSGFNEE TCLVKIITGL LEFEVYLEYL QNRFESSEEQ ARAVQMSTKV LIQFLQKKAK NLDAITTPDP TTNASLLTKL QAQNQWLQDM TTHLILRSFK EFLQSSLRAL RQM
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg mL. Stock solutions should be apportio
• Storage This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.

Interleukin-6 (IL-6) is an interleukin that in humans is encoded by the IL-6 gene and acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response. Furthermore, It plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It also induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. The human IL-6 is a single non-glycosylated polypeptide chain containing 183 amino acids and it signals through a cell-surface type I cytokine receptor complex consisting of the ligand-binding IL-6Rα chain (CD126), and the signal- transducing component gp130 (also called CD130). The human IL-6 shares about 40% a.a. sequence identity with mouse and rat IL-6 and it is equally active on mouse and rat cells.

1. Ferguson-Smith AC, Chen YF, Newman MS, et al. 1988. Genomics. 2:203-8. 2. van der Poll T, Keogh CV, Guirao X, et al. 1997. J Infect Dis. 176:439-44. 3. Ming JE, Cernetti C, Steinman RM, et al. 1989. J Mol Cell Immunol. 4:203-11; discussion 211-2. 4. Bastard JP, Jardel C, Delattre J, et al. 1999. Circulation. 99:2221-2. 5. Heinrich PC, Behrmann I, Muller-Newen G, et al. 1998. Biochem J. 334 ( Pt 2):297-314.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting