• Product NameRecombinant Human IL-8 (77a.a.)(rHu IL-8/CXCL8)
• Brief DescriptionRecombinant Protein
• Host SpeciesE.coli
• Purification> 96 % by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human CXCR2 transfected mouse BaF3 cells is less than 2 ng ml, corresponding to a specific activity of > 5.0 ⅹ 105 IU mg.
• EndotoxinLess than 1 EU/μg of rHuIL-8, 77a.a./CXCL8 as determined by LAL method.
• Target NamerHu IL-8 CXCL8
• Alternative Names    CXCL 8, Emoctakin, GCP-1, MDNCF, MONAP, NAP-1, Protein 3-10C, T-cell chemotactic factor
• Accession No. accession:P10145
  GeneID:3576
• UniprotP10145
• Gene ID3576;
• Calculated MW Approximately 8.9 kDa, a singl
• SDS-PAGE MW Sterile Filtered White lyophil
• Target Sequence AVLPRSAKEL RCQCIKTYSK PFHPKFIKEL RVIESGPHCA NTEIIVKLSD GRELCLDPKE NWVQRVVEKF LKRAENS
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg mL. Stock solutions should be apportio
• Storage This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.

Interleukin-8 (IL-8) is encoded by the IL8 gene and produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles. There are many receptors capable to bind IL-8, the most affinity to IL-8 are receptors CXCR1, and CXCR2. As a member of the CXC chemokine family, function of IL-8 is the induction of chemotaxis in its target cells, like neutrophil granulocytes, basophils, and T-cells. IL-8 (72a.a.) has a 5-10fold higher activity on neutrophil activation, compared to IL-8 (77a.a.). IL-8 is often associated with inflammation, it has been cited as a proinflammatory mediator in gingivitis and psoriasis.

1. Modi WS, Dean M, Seuanez HN, et al. 1990. Hum Genet. 84:185-7. 2. Wolff B, Burns AR, Middleton J, et al. 1998. J Exp Med. 188:1757-62. 3. Utgaard JO, Jahnsen FL, Bakka A, et al. 1998. J Exp Med. 188:1751-6. 4. Van Damme J, Rampart M, Conings R, et al. 1990. Eur J Immunol. 20:2113-8.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting