• Product NameRecombinant Human Interleukin-11(rHu IL-11)
• Brief DescriptionRecombinant Protein
• Host SpeciesE.coli
• Purification> 95 % by SDS-PAGE and HPLC analyses.
• Species ReactivityHu
• Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine B9-11 cells is less than 1 ng ml, corresponding to a specific activity of > 1.0 ⅹ 106 IU mg.
• EndotoxinLess than 1 EU/μg of rHuIL-11 as determined by LAL method.
• Target NamerHu IL-11
• Alternative Names    AGIF
• Accession No. accession:P20809
  GeneID:3589
• UniprotP20809
• Gene ID3589;
• Calculated MW Approximately 19.1 kDa, a sing
• SDS-PAGE MW Sterile Filtered White lyophil
• Target Sequence MPGPPPGPPR VSPDPRAELD STVLLTRSLL ADTRQLAAQL RDKFPADGDH NLDSLPTLAM SAGALGALQL PGVLTRLRAD LLSYLRHVQW LRRAGGSSLK TLEPELGTLQ ARLDRLLRRL QLLMSRLALP QPPPDPPAPP LAPPSSAWGG IRAAHAILGG LHLTLDWAVR GLLLLKTRL
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg mL. Stock solutions should be apportio
• Storage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Interleukin-11 (IL-11) is encoded by the IL11 gene and is a member of IL-6 superfamily, distinguished based on their use of the common co-receptor gp130. It is a multifunctional cytokine first isolated from bone marrow-derived stromal cells. IL-11 receptor activation requires formation of a complex of two IL-11 molecules with two molecules of the ligand-binding IL-11Rα subunit and two molecules of the expressed cell signaling β subunit, gp130. The functions of IL-11 are that it directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells, and induces megakaryocyte maturation resulting in increased platelet production.

1. McKinley D, Wu Q, Yang-Feng T, et al. 1992. Genomics. 13:814-9. 2. Paul SR, Bennett F, Calvetti JA, et al. 1990. Proc Natl Acad Sci U S A. 87:7512-6. 3. Barton VA, Hall MA, Hudson KR, et al. 2000. J Biol Chem. 275:36197-203.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting