• Product NameRecombinant Human Interleukin-15 (rHu IL-15)
• Brief DescriptionRecombinant Protein
• Host SpeciesE.coli
• Purification> 97 % by SDS-PAGE and HPLC analyses.
• Species ReactivityHu
• Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.5 ng ml, corresponding to a specific activity of > 2.0 ⅹ 106 IU mg.
• EndotoxinLess than 1 EU/μg of rHuIL-15 as determined by LAL method.
• Target NamerHu IL-15
• Accession No. accession:P40933
  GeneID:3600
• UniprotP40933
• Gene ID3600;
• Calculated MW Approximately 12.9 kDa, a sing
• SDS-PAGE MW Sterile Filtered White lyophil
• Target Sequence NWVNVISDLK KIEDLIQSMH IDATLYTESD VHPSCKVTAM KCFLLELQVI SLESGDASIH DTVENLIILA NNSLSSNGNV TESGCKECEE LEEKNIKEFL QSFVHIVQMF INTS
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg mL. Stock solutions should be apportio
• Storage This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.

Human Interleukin-15 (IL-15) is expressed by the IL15 gene located on the chromosome 4. It shares approximately 97 % and 73 % sequence identity with simian and murine IL-15, respectively. Both human and simian IL-15 are active on murine cells. IL-15 is secreted by mononuclear phagocytes (and some other cells), especially macrophages following infection by virus. It possesses a variety of biological functions, including stimulating and maintaining of cellular immune responses, especially regulating T and natural killer (NK) cell activation and proliferation. In additionally, it shares many biological properties with IL-2, including T, B and NK cell-stimulatory activities. IL-15 signals through a complex composed of IL-2 IL-15 receptor beta chain. Although IL-15 lacks sequence homology with IL-2, it has recently been shown that both the beta and gamma chains of the IL-2 receptor are utilized for IL-15 binding and signaling. In addition, an IL-15 specific binding protein has also been cloned from a mouse T cell clone.

1. Anderson DM, Johnson L, Glaccum MB, et al. 1995. Genomics, 25: 701-6. 2. Krause H, Jandrig B, Wernicke C, et al. 1996. Cytokine, 8: 667-74. 3. Chirifu M, Hayashi C, Nakamura T, et al. 2007. Nat Immunol, 8: 1001-7. 4. Grabstein KH, Eisenman J, Shanebeck K, et al. 1994. Science, 264: 965-8. 5. Giri JG, Ahdieh M, Eisenman J, et al. 1994. EMBO J, 13: 2822-30. 6. Arena A, Merendino RA, Bonina L, et al. 2000. New Microbiol, 23: 105-12.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting