• Product NameRecombinant Human Interleukin-17(rHu IL-17)
• Brief DescriptionRecombinant Protein
• Host SpeciesE.coli
• Purification> 95 % by SDS-PAGE and HPLC analyses.
• Species ReactivityHu
• Biological ActivityFully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion of murine NIH 3T3 cells is less than 7.5 ng ml, corresponding to a specific activity of > 1.3 ⅹ 105 IU mg.
• EndotoxinLess than 1 EU/μg of rHuIL-17 as determined by LAL method.
• Target NamerHu IL-17
• Alternative Names    Cytotoxic T-lymphocyte-associated antigen 8, CLTA-8 , IL-17A
• Accession No. accession:Q16552
  GeneID:3605
• UniprotQ16552
• Gene ID3605;
• Calculated MW Approximately 31.0 kDa, a disu
• SDS-PAGE MW Sterile Filtered White lyophil
• Target Sequence GITIPRNPGC PNSEDKNFPR TVMVNLNIHN RNTNTNPKRS SDYYNRSTSP WNLHRNEDPE RYPSVIWEAK CRHLGCINAD GNVDYHMNSV PIQQEILVLR REPPHCPNSF RLEKILVSVG CTCVTPIVHH VA
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1-1.0 mg mL. Stock solutions should be apportioned into working aliquots and stored at �� -20 ˚C
• Storage This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.

Human Interleukin-17A (IL-17A) is encoded by the IL17A gene located on the chromosome 6 and belongs to the IL-17 family that contains IL-17A, IL-17B, IL-17C, IL-17D, IL-17E and IL-17F. They have a similar protein structure, with four highly conserved cysteine residues critical to their 3-dimensional shape, but no sequence similarity to any other known cytokines. Interleukin 17 is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. Mature IL-17 containing one potential N-linked glycosylation site. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, IL-17 exhibits 63 % amino acid identity with mouse IL-17. High levels of human IL-17 were induced from primary peripheral blood CD4+ T cells upon stimulation and they can induce stromal cells to produce proinflammatory and hematopoietic cytokines.

1. Mungall AJ, Palmer SA, Sims SK, et al. 2003. Nature, 425: 805-11. 2. Kolls JKandLinden A. 2004. Immunity, 21: 467-76. 3. Fossiez F, Djossou O, Chomarat P, et al. 1996. J Exp Med, 183: 2593-603. 4. Yao Z, Painter SL, Fanslow WC, et al. 1995. J Immunol, 155: 5483-6.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting