• Product NameRecombinant Human Interleukin-21(rHu IL-21)
• Brief DescriptionRecombinant Protein
• Host SpeciesE.coli
• Purification> 97 % by SDS-PAGE and HPLC analyses.
• Species ReactivityHu
• Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human N1186 T cells is less than 20 ng ml, corresponding to a specific activity of > 5.0 ⅹ 104 IU mg.
• EndotoxinLess than 1 EU/μg of rHuIL-21 as determined by LAL method.
• Target NamerHu IL-21
• Alternative Names    Za11
• Accession No. accession:Q9HBE4
  GeneID:59067
• UniprotQ9HBE4
• Gene ID59067;
• Calculated MW Approximately 15.4 kDa, a sing
• SDS-PAGE MW Sterile Filtered White lyophil
• Target Sequence QGQDRHMIRM RQLIDIVDQL KNYVNDLVPE FLPAPEDVET NCEWSAFSCF QKAQLKSANT GNNERIINVS IKKLKRKPPS TNAGRRQKHR LTCPSCDSYE KKPPKEFLER FKSLLQKMIH QHLSSRTHGS EDS
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg mL. Stock solutions should be apportio
• Storage This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.

Human IL-21 is encoded by IL-21 gene located on Chr. 4. It is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation and can regulating immune system cells, for instance cytotoxin T cells and natural killer cells. Additionally, it can induce target cells division or proliferation. IL-21 elicits its effect through binding to IL-21R, which also contains the gamma chain found in other cytokine receptors such as IL-2, IL-4, IL-7, IL-9 and IL-15. IL-21 IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. IL-21 shows having much relation with clinical illnesses, including cancer immunotherapy, viral infections and allergies.

1. Pistoia VandCocco C. 2009. J Leukoc Biol, 85: 739-43. 2. Ertelt JM, Johanns TM, Rowe JH, et al. 2010. Immunology, 131: 183-91. 3. Denman CJ, Senyukov VV, Somanchi SS, et al. 2012. PLoS One, 7: e30264. 4. Spolski R, Wang L, Wan CK, et al. 2012. J Immunol, 188: 1924-32.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting