• Product NameRecombinant Human Interleukin-31(rHu IL-31)
• Brief DescriptionRecombinant Protein
• Host SpeciesE.coli
• Purification> 97 % by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. The specific activity is determined by inducing STAT3 activation using human U-87 MG cells. 5 ng mL of rHuIL-31 can effectively induce STAT3 activation.
• EndotoxinLess than 1 EU/μg of rHuIL-31 as determined by LAL method.
• Target NamerHu IL-31
• Accession No. accession:Q6EBC2
  GeneID:386653
• UniprotQ6EBC2
• Gene ID386653;
• Calculated MW Approximately 15.8 kDa, a sing
• SDS-PAGE MW Sterile Filtered White lyophil
• Target Sequence SHTLPVRLLR PSDDVQKIVE ELQSLSKMLL KDVEEEKGVL VSQNYTLPCL SPDAQPPNNI HSPAIRAYLK TIRQLDNKSV IDEIIEHLDK LIFQDAPETN ISVPTDTHEC KRFILTISQQ FSECMDLALK SLTSGAQQAT T
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4.
• Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg mL. Stock solutions should be apportio
• Storage This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.

Human IL-31 gene is located on Chr.12. It expresses the IL-31 protein at low levels in the type 2 helper T cells, which exits in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea. This protein shares several structural and functional characteristics with IL-6, Oncostatin M, LIF, and Cardiotrophin-1. IL-31 signals through IL-31 receptor A and oncostatin M receptor subunits and can activate STAT3 through receptors and maybe involve in skin immunity. It regulated immune responses have been implicated in skin physiology and inflammatory skin diseases. Human IL-31 shares 24 % a.a. sequence identity in the mature protein with mouse IL-31.

1. Sonkoly E, Muller A, Lauerma AI, et al. 2006. J Allergy Clin Immunol, 117: 411-7. 2. Jawa RS, Chattopadhyay S, Tracy E, et al. 2008. J Interferon Cytokine Res, 28: 207-19. 3. Takaoka A, Arai I, Sugimoto M, et al. 2005. Eur J Pharmacol, 516: 180-1. 4. Ohmatsu H, Sugaya M, Suga H, et al. 2012. Acta Derm Venereol, 92: 282-3

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting