• Product NameRecombinant Human Interleukin-33(rHu IL-33)
• Brief DescriptionRecombinant Protein
• Host SpeciesE.coli
• Purification> 97 % by SDS-PAGE and HPLC analyses.
• Species ReactivityHu
• Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.05 ng ml, corresponding to a specific activity of > 2.0 ⅹ 107 IU mg.
• EndotoxinLess than 1 EU/μg of rHuIL-33 as determined by LAL method.
• Target NamerHu IL-33
• Alternative Names    IL-1F11, NF-HEV, DVS 27
• Accession No. accession:O95760
  GeneID:90865
• UniprotO95760
• Gene ID90865;
• Calculated MW Approximately 17.9 kDa, a sing
• SDS-PAGE MW Sterile Filtered White lyophil
• Target Sequence SITGISPITE YLASLSTYND QSITFALEDE SYEIYVEDLK KDEKKDKVLL SYYESQHPSN ESGDGVDGKM LMVTLSPTKD FWLHANNKEH SVELHKCEKP LPDQAFFVLH NMHSNCVSFE CKTDPGVFIG VKDNHLALIK VDSSENLCTE NILFKLSET
• Formulation Lyophilized from a 0.2μm filtered concentrated solution in 20 mM PB, 150 mM NaCl, 1mM EDTA, pH7.4.
• Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg mL. Stock solutions should be apportio
• Storage This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.

IL-33, encoded by IL-33 gene located on the Chr.9 in humans, is a 30 kDa proinflammatory protein belonging to the IL-1 superfamily and it shares less than 20 % a.a. sequence identity with other members. IL-33 secreted by high endothelial venules at high levels, which is found in tonsils, peyer patches and mesenteric lymph nodes, but not in placenta. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL1α or IL1β stimulation. It elicits its biological effects by interacting with IL1RL1 ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3 ERK1 and or MAPK1 ERK2, MAPK14, and MAPK8. IL-33 mature protein has 52-58 % a.a. sequence identity with mouse and rat IL-33.

1. Liew FY, Pitman NI, McInnes IB. 2010. Nat Rev Immunol, 10: 103-10. 2. Miller AM, Xu D, Asquith DL, et al. 2008. J Exp Med, 205: 339-46. 3. Bourgeois E, Van LP, Samson M, et al. 2009. Eur J Immunol, 39: 1046-55. 4. Zhiguang X, Wei C, Steven R, et al. 2010. Immunol Lett, 131: 159-65. 5. Turnquist HR, Zhao Z, Rosborough BR, et al. 2011. J Immunol, 187: 4598-610.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting