• Product NameGATA1(Ab-142) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total GATA1 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.140~144 (R-L-S-P-D) derived from Human GATA1.
• Target NameGATA1
• Alternative Names    GAT1; GATA1; GF-1; NF-E1;
• Accession No. Swiss-Prot: P15976
  NCBI Protein: NP_002040.1
• UniprotP15976
• Gene ID2623;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 43kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

GATA1 encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia.

Zon LI, et al. (1990) Proc Natl Acad. Sci USA. 87: 668-672.
Trainor C D, et al. (1990) Nature. 343: 92-96.
Nichols K E, et al. (2000) Nat Genet. 24: 266-270.
Freson K, et al. (2001) Blood. 98: 85-92.
Mehaffey M G, et al. (2001) Blood. 98: 2681-2688.

• Western blot analysis of extracts from 3T3 cells using GATA1(Ab-142) Antibody #21041.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GATA1(Ab-142) Antibody #21041(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed K562 cells using GATA1 (Ab-142) Antibody #21041.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting