• Product NameRecombinant Human Granulocyte Macrophage Colony Stimlating Factor (rHu GM-CSF)
• Brief DescriptionRecombinant Protein
• Host SpeciesE.coli
• Purification> 98 % by SDS-PAGE and HPLC analyses.
• Species ReactivityHu
• Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng ml, corresponding to a specific activity of > 1.0 ⅹ 107 IU mg.
• EndotoxinLess than 1.0 EU/ag of rHuGM-CSF as determined by LAL method.
• Target NamerHu GM-CSF
• Alternative Names    Granulocyte Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-α
• Accession No. accession:P04141
  GeneID:1437
• UniprotP04141
• Gene ID1437;
• Calculated MW Approximately 14.5 kDa, a sing
• SDS-PAGE MW Sterile Filtered White lyophil
• Target Sequence APARSPSPST QPWEHVNAIQ EARRLLNLSR DTAAEMNETV EVISEMFDLQ EPTCLQTRLE LYKQGLRGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFESFKENL KDFLLVIPFD CWEPVQE
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg mL. Stock solutions should be apportio
• Storage This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.

Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is secreted by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimulation. It was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors and has functions of stimulates the growth and differentiation of hematopoietic precursor cells from various lineages. GM-CSF has also been reported to have a functional role on non-hematopoietic cells and can induce human endothelial cells to migrate and proliferate. Additionally, it can stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. Human GM-CSF shares 54 % sequences identity with mouse GM-CSF, but has no biological effects across species. GM-CSF is used as a medication to stimulate the production of white blood cells following chemotherapy and has also recently been evaluated in clinical trials for its potential as a vaccine adjuvant in HIV-infected patients.

1. Wang JM, Chen ZG, Colotta F, et al. 1988. Behring Inst Mitt: 270-3. 2. 1989. N Engl J Med, 320: 253-4. 3. Nissen-Druey C. 1989. Nouv Rev Fr Hematol, 31: 99-101. 4. Eager RandNemunaitis J. 2005. Mol Ther, 12: 18-27. 5. Tran T, Fernandes DJ, Schuliga M, et al. 2005. Br J Pharmacol, 145: 123-31.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting