• Product NameRecombinant Human Flt-3 Ligand (rHuFlt-3L)
• Brief DescriptionRecombinant Protein
• Host SpeciesE.coli
• Purification> 97 % by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human AML5 cells is less than 1.0 ng ml, corresponding to a specific activity of > 1.0 ⅹ 106 IU mg.
• EndotoxinLess than 1 EU/ag of rHuFlt3-Ligand as determined by LAL method.
• Target NamerHuFlt-3L
• Alternative Names    Flt3L, SL Cytokine
• Accession No. accession:P49771
  GeneID:2323
• UniprotP49771
• Gene ID2323;
• Calculated MW Approximately 17.6 kDa, a sing
• SDS-PAGE MW Sterile Filtered White lyophil
• Target Sequence TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.
• Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg mL. Stock solutions should be apportio
• Storage This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.

Flt3-ligand (FL) is a recently identified hematopoietic cytokine whose activities are mediated by binding to the transmembrane glycoprotein Flt3. Flt3 was first discovered as a member of the class III subfamily of receptor tyrosine kinases (RTK) whose expression among hematopoietic cells was found to be restricted to highly enriched stem progenitor cell populations. Additionally, class III RTKs include the receptors from SCF, M-CSF and PDGF. Not surprisingly, Flt3-ligand is also structurally related to M-CSF and SCF. All three cytokines have been shown to exist both as type I transmembrane proteins and as soluble proteins. The predominant human FL isoform is a transmembrane protein that can undergo proteolytic cleavage to generate a soluble form of the protein. An alternatively-spliced FL mRNA, encoding a soluble form of the human FL, has also been identified. FL is widely expressed in various human and mouse tissues. At the amino acid sequence level, human and mouse FL are approximately 72 % identical and the two proteins exhibit cross-species activity. FL has been shown to synergize with a wide variety of hematopoietic cytokines to stimulate the growth and differentiation of early hematopoietic progenitors.

1. Hacein-Bey S, Basile GD, Lemerle J, et al. 1998. Blood, 92: 4090-7. 2. Peters M, Solem F, Goldschmidt J, et al. 2001. Exp Hematol, 29: 146-55. 3. Beq S, Fontanet A, Theze J, et al. 2004. AIDS, 18: 2089-91. 4. Mahadevan D, Choi J, Cooke L, et al. 2009. Hum Genomics Proteomics, 2009: 453634.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting