• Product NameRecombinant Human soluble CD40 Ligand (rHu sCD40L)
• Brief DescriptionRecombinant Protein
• Host SpeciesE.coli
• Purification> 95 % by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 5-10 ng ml.
• EndotoxinLess than 1 EU/ag of rHusCD40L as determined by LAL method.
• Target NamerHu sCD40L
• Alternative Names    TNFSF5, sCD40L, CD154, gp39, T-BAM, TNF-related Activation Protein, TRAP
• Accession No. accession:P29965
  GeneID:959
• UniprotP29965
• Gene ID959;
• Calculated MW Approximately 16.3 kDa, a sing
• SDS-PAGE MW Sterile Filtered White lyophil
• Target Sequence MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.
• Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg mL. Stock solutions should be apportio
• Storage This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.

CD40 ligand is a 261 amino acid type II transmembrane glycoprotein belonging to the TNF family and can be cleaved into two chains: the CD40 ligand membrane form and CD40 ligand soluble form. It is primarily expressed on activated CD4+ T lymphocytes, and also found in other types of cells, like NK cells, mast cells, basophils and eosinophils. In total CD40 ligand has three binding partners: CD40, α5β1 integrin and αIIbβ3. It mediates a range of activities on B cells including induction of activation-associated surface antigen, entry into cell cycle, isotype switching and Ig secretion and memory generation. CD40-CD40L interaction also plays important roles in monocyte activation and dendritic cell maturation.

1. Li R, Chen WC, Pang XQ, et al. 2011. Mol Biol Rep, 38: 5459-64. 2. Reinboldt S, Wenzel F, Rauch BH, et al. 2009. Platelets, 20: 441-4. 3. Tousoulis D, Antoniades C, Nikolopoulou A, et al. 2007. Eur J Clin Invest, 37: 623-8. 4. Varo N, Libby P, Nuzzo R, et al. 2005. Diab Vasc Dis Res, 2: 81-7. 5. Holzer G, Pfandlsteiner T, Blahovec H, et al. 2003. Wien Med Wochenschr, 153: 40-2.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting