• Product NameRecombinant Human Oncostatin-M (rHu OSM)
• Brief DescriptionRecombinant Protein
• Host SpeciesE.coli
• Purification> 95 % by SDS-PAGE and HPLC analyses.
• Species ReactivityHu
• Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 2 ng ml, corresponding to a specific activity of > 5.0 ⅹ 105 IU mg.
• EndotoxinLess than 1 EU/ag of rHuOSM as determined by LAL method.
• Target NamerHu OSM
• Alternative Names    Oncostatin M
• Accession No. accession:P13725
  GeneID:5008
• UniprotP13725
• Gene ID5008;
• Calculated MW Approximately 25.8 kDa, a sing
• SDS-PAGE MW Sterile Filtered White lyophil
• Target Sequence AAIGSCSKEY RVLLGQLQKQ TDLMQDTSRL LDPYIRIQGL DVPKLREHCR ERPGAFPSEE TLRGLGRRGF LQTLNATLGC VLHRLADLEQ RLPKAQDLER SGLNIEDLEK LQMARPNILG LRNNIYCMAQ LLDNSDTAEP TKAGRGASQP PTPTPASDAF QRKLEGCRFL HGYHRFMHSV GRVFSKWGES PNRSRRHSPH QALRKGVRRT RPSRKGKRLM TRGQLPR
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg mL. Stock solutions should be apportio
• Storage This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.

Oncostatin M (OSM) is a multifunctional cytokine that belongs to the Interleukin-6 subfamily. Among the family members, OSM is most closely related to leukemia inhibitory factor (LIF) and it in fact utilizes the LIF receptor in addition to its specific receptor in the human. A biologically active OSM receptor has been previously described that consists of a heterodimer of leukemia inhibitory factor receptor (LIFR) and gp130. OSM is synthesized by stimulated T-cells and monocytes. The effects of OSM on endothelial cells suggest a pro-inflammatory role for OSM and endothelial cells possess a large number of OSM receptors. Recombinant murine OSM contains 181 amino acids and has a molecular mass of 20.4 kDa. It has approximately 48 % and 72 % amino acid sequence identity with human and rat OSM.

1. Tanaka M, Miyajima A. 2003. Rev Physiol Biochem Pharmacol. 149:39-52. 2. Mosley B, De Imus C, Friend D, et al. 1996. J Biol Chem. 271:32635-43. 3. Malik N, Kallestad JC, Gunderson NL, et al. 1989. Mol Cell Biol. 9:2847-53. 4. Brown TJ, Rowe JM, Liu JW, et al. 1991. J Immunol. 147:2175-80.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting