• Product NameRecombinant Human BMP-4
• Brief DescriptionRecombinant Protein
• Host SpeciesE.coli
• Purification> 95 % by SDS-PAGE and HPLC analyses.
• Species ReactivityHu
• EndotoxinLess than 1 EU/μg of rHuBMP-4 as determined by LAL method.
• Target NamerHu BMP-4
• Alternative Names    BMP-2B
• Accession No. accession:P12644
  GeneID:652
• UniprotP12644
• Gene ID652;
• Calculated MW Approximately 13.3 kDa, a mono
• SDS-PAGE MW Sterile Filtered White lyophil
• Target Sequence MSPKHHSQRA RKKNKNCRRH SLYVDFSDVG WNDWIVAPPG YQAFYCHGDC PFPLADHLNS TNHAIVQTLV NSVNSSIPKA CCVPTELSAI SMLYLDEYDK VVLKNYQEMV VEGCGCR
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 50 mM Na2CO3, 5 mM DTT, pH 11.0.
• Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg mL. Stock solutions should be apportioned into working aliquots and stored at
• Storage This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.

Bone Morphogenetic Protein 4 is one of the BMPs, some of which belong to the TGF-beta superfamily (BMP2-7). There are more than thirteen BMPs have been discovered nowadays and they are involved in inducing cartilage and bone formation. BMP-4 is expressed in the lung and lower levels seen in the kidney. It also presents in normal and neoplastic prostate tissues, and prostate cancer cell lines. It regulates the formation of teeth, limbs and bone from mesoderm. Furthermore it also plays a role in fracture repair. BMP-4 signals through tetrameric complexes composed of type I and type II receptors and regulates it function by interaction with multiple proteins and glycosaminoglycans. The human BMP-4 shares 98 % sequence indentity with mouse BMP-4. Reduced expression of BMP-4 is associated with a number of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva.

1. Yamashita H, Murayama C, Takasugi R, et al. 2011. Mol Cell Biochem, 348: 183-90. 2. Yates KE, Troulis MJ, Kaban LB, et al. 2002. Int J Oral Maxillofac Surg, 31: 173-8. 3. Bessa PC, Cerqueira MT, Rada T, et al. 2009. Protein Expr Purif, 63: 89-94. 4. Kawakami T, Kumasaka M, Kato M, et al. 2011. J Dermatol Sci, 63: 66-9.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting