- Product NameRecombinant murine Interleukin-1 b (rm IL-1b )
- Brief DescriptionRecombinant Protein
- Host SpeciesE.coli
- Purification> 96 % by SDS-PAGE and HPLC analyses.
- Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 2 pg ml, corresponding to a specific activity of > 5.0 ⅹ 108 IU mg.
- EndotoxinLess than 1 EU/μg of rMuIL-1a as determined by LAL method.
- Target Namerm IL-1b
- Alternative Names Catabolin, Leukocyte Endogenous Mediator, LEM, Lymphocyte-activating factor , LAF, Mononuclear Cell Factor, MCF , Endogenous Pyrogen, EP
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Accession No. accession:P10749
GeneID:16176 - UniprotP10749
- Gene ID16176;
- Calculated MW Approximately 17.5 kDa, a sing
- SDS-PAGE MW Sterile Filtered White lyophil
- Target Sequence VPIRQLHYRL RDEQQKSLVL SDPYELKALH LNGQNINQQV IFSMSFVQGE PSNDKIPVAL GLKGKNLYLS CVMKDGTPTL QLESVDPKQY PKKKMEKRFV FNKIEVKSKV EFESAEFPNW YISTSQAEHK PVFLGNNSGQ DIIDFTMESV SS
- Formulation Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4, with 5 % trehalose, 0.02 % Tween-20.
- Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg mL. Stock solutions should be apportio
- Storage This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.
