• Product NameRecombinant murine Interleukin-2 (rm IL-2 )
• Brief DescriptionRecombinant Protein
• Host SpeciesE.coli
• Purification> 95 % by SDS-PAGE and HPLC analyses.
• Species ReactivityMs
• Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.2 ng ml, corresponding to a specific activity of > 5.0 ⅹ 106 IU mg.
• EndotoxinLess than 1 EU/μg of rMuIL-2 as determined by LAL method.
• Target Namerm IL-2
• Alternative Names    T-cell growth factor, TCGF, Aldesleukin
• Accession No. accession:P04351
  GeneID:16183
• UniprotP04351
• Gene ID16183;
• Calculated MW Approximately 17.2 kDa, a sing
• SDS-PAGE MW Sterile Filtered White lyophil
• Target Sequence APTSSSTSSS TAEAQQQQQQ QQQQQQHLEQ LLMDLQELLS RMENYRNLKL PRMLTFKFYL PKQATELKDL QCLEDELGPL RHVLDLTQSK SFQLEDAENF ISNIRVTVVK LKGSDNTFEC QFDDESATVV DFLRRWIAFC QSIISTSPQ
• Formulation Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
• Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg mL. Stock solutions should be apportio
• Storage This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2 IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL2 consists of three subunits (55 kDa IL2Rα, 75 kDa IL2Rβ, 64 kDa common gamma chain γc IL2Rγ) that are present on the cell surface in varying preformed complexes; Mature mouse IL2 shares 56 % and 73 % amino acid sequence identity with human and rat IL2 respectively. Mouse and human IL2 exhibit cross-species activity. Mouse IL2 has a specific N-terminal region that contains a poly glutamine (contain 12 glutamines) stretch.

1. Ma, A., R. Koka, and P. Burkett. 2006. Annu Rev Immunol, 24: 657-79. 2. Taniguchi, T., H. Matsui, T. Fujita, et al. 1983. Nature, 302: 305-10. 3. Liparoto, S.F., D.G. Myszka, Z. Wu, et al. 2002. Biochemistry, 41: 2543-51. 4. Bodnar, A., E. Nizsaloczki, G. Mocsar, et al. 2008. Immunol Lett, 116: 117-25. 5. Mosmann, T.R., T. Yokota, R. Kastelein, et al. 1987. J Immunol, 138: 1813-6. 6. Matesanz, F., A. Alcina, and A. Pellicer. 1993. Immunogenetics, 38: 300-3.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting