• Product NameRecombinant murine Interleukin-4 (rm IL-4 )
• Brief DescriptionRecombinant Protein
• Host SpeciesE.coli
• Purification> 97 % by SDS-PAGE and HPLC analyses.
• Species ReactivityMs
• Biological ActivityFully biologically active when compared to standard. The ED50 as determined by the dose-dependant prolifiration of Murine HT-2 cells is less then 2 ng ml, corresponding to a Specific Activity of > 5 ⅹ 105 IU mg.
• EndotoxinLess than 1 EU/μg of rMuIL-4 as determined by LAL method.
• Target Namerm IL-4
• Alternative Names    B-cell IgG differentiation factor, B-cell growth factor 1, BSF-1, IGG1 induction factor, Lymphocyte stimulatory factor 1
• Accession No. accession:P07750
  GeneID:16189
• UniprotP07750
• Gene ID16189;
• Calculated MW Approximately 13.5 kDa, a sing
• SDS-PAGE MW Sterile Filtered White lyophil
• Target Sequence MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S
• Formulation Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
• Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg mL. Stock solutions should be apportio
• Storage This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.

Interleukin-4 (IL-4) is a pleiotropic cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. It is produced by mast cells, activated T cells and bone marrow stromal cells. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. In addition, IL-4 enhances both secretion and cell surface expression of IgE and IgG1 and also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. The mouse IL-4 has a compact, globular fold, stabilised by 3 disulphide bonds and contains 121 amino acids residues which is a single non-glycosylated polypeptide. The human IL-4 shares about 40% aa sequence identity with mouse rat IL-4 and they are species-specific in their activities.

1. Sokol CL, Barton GM, Farr AG, et al. 2008. Nat Immunol. 9:310-8. 2. Hershey GK, Friedrich MF, Esswein LA, et al. 1997. N Engl J Med. 337:1720-5. 3. Yokota T, Otsuka T, Mosmann T, et al. 1986. Proc Natl Acad Sci U S A. 83:5894-8. 4. Eder A, Krafft-Czepa H, Krammer PH. 1988. Nucleic Acids Res. 16:772. 5. Walter MR, Cook WJ, Zhao BG, et al. 1992. J Biol Chem. 267:20371-6

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting