• Product NameRecombinant murine Vascular Endothelial Growth Factor 120 (rm VEGF120)
• Brief DescriptionRecombinant Protein
• Host SpeciesE.coli
• Purification> 96 % by SDS-PAGE and HPLC analyses.
• Biological ActivityFully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is less than 5 ng ml, corresponding to a specific activity of > 2.0 ⅹ 105 IU mg.
• EndotoxinLess than 1 EU/ag of rMuVEGF120 as determined by LAL method.
• Target Namerm VEGF120
• Alternative Names    Vascular endothelial growth factor isoform 120
• Accession No. accession:Q00731
  GeneID:Mm.282184.
• UniprotQ00731
• Gene ID22339;
• Calculated MW Approximately 28.4 kDa, a disu
• SDS-PAGE MW Sterile Filtered White lyophil
• Target Sequence MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKCDKPR R
• Formulation Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
• Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg mL. Stock solutions should be apportio
• Storage This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.

Vascular Endothelial Growth Factor is a sub-family of growth factors produced by cells that stimulates vasculogenesis and angiogenesis. VEGF's normal function is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels. VEGF signals through the three receptors: fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Mouse express alternately spliced isoforms of 120, 164, 182 amino acids (a.a.) in length. The VEGF120 shares 98 % a.a. sequence identity with corresponding regions of rat, 89 % with canine, feline, equine and porcine, and 87 % with human, ovine and bovine VEGF, respectively.

1. Leung DW, Cachianes G, Kuang WJ, et al. 1989. Science. 246:1306-9. 2. Byrne AM, Bouchier-Hayes DJ, Harmey JH. 2005. J Cell Mol Med. 9:777-94. 3. Robinson CJ, Stringer SE. 2001. J Cell Sci. 114:853-65.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting