• Product NameRecombinant Rat Interferon-g (rRa IFN-g)
• Brief DescriptionRecombinant Protein
• Host SpeciesE.coli
• Purification> 97 % by SDS-PAGE and HPLC analyses.
• Species ReactivityRt
• Biological ActivityFully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using murine L929 cells infected with encephalomyocarditis (EMC) virus is less than 0.1 ng ml, corresponding to a specific activity of > 1.0 ⅹ 107 IU m
• EndotoxinLess than 0.1 EU/μg of rRtIFN-a as determined by LAL method.
• Target Namerr IFN-g
• Accession No. accession:P01581
  GeneID:25712
• UniprotP01581
• Gene ID25712;
• Calculated MW Approximately 15.5 kDa, a sing
• SDS-PAGE MW Sterile Filtered White lyophil
• Target Sequence QGTLIESLES LKNYFNSSSM DAMEGKSLLL DIWRNWQKDG NTKILESQII SFYLRLFEVL KDNQAISNNI SVIESHLITN FFSNSKAKKD AFMSIAKFEV NNPQIQHKAV NELIRVIHQL SPESSLRKRK RSRC
• Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 1 μ PBS, pH 7.4, 1 mM DTT, 5 % Trehalose and 0.05 % Tween-80.
• Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HAc to a concentration of 0.1-1.0 mg mL. Stock solutions should be apportioned into working aliquots and stored at �� -20 ˚
• Storage This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.

Interferon-gamma (IFN-γ), also known as Type II interferon or immune interferon, is a cytokine produced primarily by T-lymphocytes and natural killer cells. The protein shares no significant homology with IFN-β or the various IFN-α family proteins. Mature IFN-γ exists as noncovalently-linked homodimers. It shares high sequence indentity with mouse IFN-γ (86 %). IFN-γ was originally characterized based on its antiviral activities. The protein also exerts antiproliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-γ induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. Additionally, IFN-γ augments TH1 cell expansion and may be required for TH1 cell differentiation.

1. Wang H, Ruan Z, Wang Y, et al. 2008. Mol Immunol, 45: 1548-56. 2. Kopinski P, Przybylski G, Jarzemska A, et al. 2007. Pol Merkur Lekarski, 23: 15-21. 3. Pennino D, Bhavsar PK, Effner R, et al. 2012. J Allergy Clin Immunol, 4. Hibi M, Hachimura S, Ise W, et al. 2003. Cytotechnology, 43: 49-55.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting