• Product NameRecombinant Rat SDF-1 a (rRt SDF-1a/CXCL12 )
• Brief DescriptionRecombinant Protein
• Host SpeciesE.coli
• Purification> 97 % by SDS-PAGE and HPLC analyses.
• Species ReactivityRt
• Biological ActivityFully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 50-100 ng ml.
• EndotoxinLess than 1 EU/��g of rRtSDF-1��/CXCL12�� as determined by LAL method.
• Target Namerr SDF-1a CXCL12
• Accession No. accession:Q9QZD1
  GeneID:24772
• UniprotQ9QZD1
• Gene ID24772;
• Calculated MW Approximately 7.9 kDa, a singl
• SDS-PAGE MW Sterile Filtered White lyophil
• Target Sequence KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNK
• Formulation Lyophilized from a 0.2 ��m filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
• Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg mL. Stock solutions should be apportio
• Storage This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.

CXCL12 also known as SDF-1 is belonging to the CXC chemokine family. It is encoded by the CXCL12 gene. Rat CXCL12 is expressed as two isoforms that differ only in the C-terminal tail. And both SDF-1 isoforms undergo proteolytic processing of the first two N-terminal amino acids. Contrast to SDF-1β, SDF-1α is shorter by four amino acids at the C-terminal tail. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. SDF-1 is highly conserved between species, rat CXCL12α shares approximately 96% amino acid sequence identity with human CXCL12α.

1. Shirozu M, Nakano T, Inazawa J, et al. 1995. Genomics. 28:495-500. 2. De La Luz Sierra M, Yang F, Narazaki M, et al. 2004. Blood. 103:2452-9. 3. Charnaux N, Brule S, Hamon M, et al. 2005. FEBS J. 272:1937-51. 4. Bleul CC, Fuhlbrigge RC, Casasnovas JM, et al. 1996. J Exp Med. 184:1101-9. 5. Ara T, Nakamura Y, Egawa T, et al. 2003. Proc Natl Acad Sci U S A. 100:5319-23. 6. Askari AT, Unzek S, Popovic ZB, et al. 2003. Lancet. 362:697-703. 7. Ma Q, Jones D, Borghesani PR, et al. 1998. Proc Natl Acad Sci U S A. 95:9448-53.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting