• Product NameBCL-2(Ab-56) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total BCL-2 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 54~58 (G-H-T-P-H) derived from Human BCL-2.
• Target NameBCL-2
• Alternative Names    BCL2
• Accession No. Swiss-Prot: P10415
  NCBI Protein: NP_000624.2
• UniprotP10415
• Gene ID596;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 26kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

Ling, Y. H. et al. (1998) J. Biol. Chem. 273, 18984-18991.
Huang, S.J. and Cidlowski, J.A. (2002) FASEB 16, 825-832.
Deng, X. et al. (2001) J. Biol. Chem. 276, 23681-23688.
Huang ST,et al. (2002) FASEB J Jun; 16(8): 825-32.
Yamamoto, K. et al. (1999) Mol. Cell. Biol. 19, 8469-8478.

• Western blot analysis of extracts from Hela, A2780 and 293 cells using BCL-2(Ab-56) Antibody #21059.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BCL-2(Ab-56) Antibody #21059(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed Hela cells using BCL-2(Ab-56) Antibody #21059.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting