• Product NameBCL-2(Ab-70) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total BCL-2 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.68~72 (R-T-S-P-L) derived from Human BCL-2.
• Target NameBCL-2
• Alternative Names    BCL2
• Accession No. Swiss-Prot: P10415
  NCBI Protein: NP_000624.2
• UniprotP10415
• Gene ID596;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 26kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

BCL-2 encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.

Muller IM, et al. (2005) Mol Pharmacol.
Jin Z, Gao F,et al. J Biol Chem 2004 Sep 17; 279(38): 40209-19.
Kumar Biswas S,et al. Mol Cancer Ther 2004 Mar; 3(3): 327-34.
Huang ST,et al. (2002)FASEB J; 16(8): 825-32.
Yamamoto K,et al. (1999) Mol Cell Biol; 19(12): 8469-78.

• Western blot analysis of extracts from MCF cells using BCL-2(Ab-70) Antibody #21060 and the same antibody preincubated with blocking peptide.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BCL-2(Ab-70) Antibody #21060(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed Hela cells using BCL-2(Ab-70) Antibody #21060.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting