• Product NameBAD(Ab-112) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total BAD protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.110~114 (H-S-S-Y-P) derived from Mouse BAD.
• Target NameBAD
• Alternative Names    Bbc2
• Accession No. Swiss-Prot: Q61337
  NCBI Protein: NP_031548.1
• UniprotQ61337
• Gene ID12015;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 23kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

Zhang B, et al. (2004). Mol Cell Biol.24 (14): 6205-6214.
Rice PL, et al. (2003). Cancer Res.63 (3): 616-620.
Wang XQ, et al. (2001). J Biol Chem.276 (48): 44504-44511.

• Western blot analysis of extracts from 293 cells using BAD(Ab-112) Antibody #21062 and the same antibody preincubated with blocking peptide.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD(Ab-112) Antibody #21062(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting