• Product NameEstrogen Receptor-a(Ab-104) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Estrogen Receptor-a protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.102~106 (S-V-S-P-S) derived from Human Estrogen Receptor-a.
• Target NameEstrogen Receptor-a
• Alternative Names    ER; ESR; ESR1; ESTR; ESTRA
• Accession No. Swiss-Prot: P03372
  NCBI Protein: NP_000116.2
• UniprotP03372
• Gene ID2099;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 66kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.

Medunjanin S, et al. (2005). J Biol Chem.80 (38):33006-33014.
Dutertre M, et al. (2003). Mol Endocrinol.17 (7): 1296-1314.
Chen D, et al. (2000). Mol Cell.6 (1): 127-137.
Rogatsky I, et al. (1999). J Biol Chem.274 (32): 22296-22302.

• Western blot analysis of extracts from MCF-7
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-a(Ab-104) Antibody #21065(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting