• Product NameEstrogen Receptor-a(Ab-167) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Estrogen Receptor-a protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.165~169 (L-A-S-T-N) derived from Human Estrogen Receptor-a.
• Target NameEstrogen Receptor-a
• Alternative Names    ER; ESR; ESR1; ESTR; ESTRA
• Accession No. Swiss-Prot: P03372
  NCBI Protein: NP_000116.2
• UniprotP03372
• Gene ID2099;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 66kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.

H Yamaguchi, J Zhu, T Yu, et al. (2007) Serum-free mouse embryo cells generate a self-sustaining feedback loop for an astrocyte marker protein and respond to cytokines and bisphenol A in accordance with the subtle difference in their differentiation state. Cell Biology International, 31(6):638-644.
This article references the use of the #21068 in the following applications :IF-IC

• Western blot analysis of extracts from MCF cells using Estrogen Receptor-a(Ab-167) Antibody #21068 and the same antibody preincubated with blocking peptide.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-a(Ab-167) Antibody #21068(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed MCF cells using Estrogen Receptor-a(Ab-167) Antibody #21068.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting