• Product NameHER2(Ab-1248) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total HER2 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 1246~1250 (P-E-Y-L-G) derived from Human HER2.
• Target NameHER2
• Alternative Names    C-erbB-2; ErbB2;
• Accession No. Swiss-Prot: P04626
  NCBI Protein: NP_001005862.1
• UniprotP04626
• Gene ID2064;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 185kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-a and amphiregulin.

Ramsauer VP, et al. (2003)J Biol Chem; 278(32): 30142-7.
Jepson S, et al. (2002)Oncogene; 21(49): 7524-32.
Ren Z, et al. (2002)J Biol Chem; 277(41): 38486-93.
Eppenberger-Castori S, et al. (2001)J Clin Oncol; 19(3): 645-56.

• Western blot analysis of extracts from MCF and SKOV3 cells using HER2(Ab-1248) Antibody #21072.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HER2(Ab-1248) Antibody #21072(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed MCF7 cells using HER2(Ab-1248) Antibody #21072.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting