• Product NameEGFR Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total EGFR protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide derived from Human EGFR.
• Target NameEGFR
• Alternative Names    Receptor tyrosine-protein kinase ErbB-1; ERBB1;
• Accession No. Swiss-Prot: P00533
  NCBI Protein: NP_005219.2
• UniprotP00533
• Gene ID1956;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 175kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentia

Feinmesser RL,et al. (1999) J Biol Chem; 274(23): 16168-73.
Gamou S,et al. (1995)FEBS Lett; 357(2): 161-4.
Gamou S, et al. (1994)J Cell Physiol; 158(1): 151-9.
Heisermann GJ, et al. (1988) J Biol Chem; 263(26): 13152-8.

• Western blot analysis of extracts from HUVEC and MDA cells using EGFR(Ab-1070) Antibody #21073.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR(Ab-1070) Antibody #21073(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed MCF cells using EGFR(Ab-1070) Antibody #21073.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting