• Product NameHistone H3 (Di-Methyl-Lys27) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around Di-Methylation site of lysine 27(A-R-K(di-methyl)-S-A) derived from Human Histone H3.
• Target NameHistone H3
• ModificationMethyl
• Alternative Names    H3/a, H3/c, H3/d, H3/f, H3/h
• Accession No. Swiss-Prot#:P68431 NCBI Gene#:8351 NCBI Protein#:NP_003521.2
• UniprotP68431
• Gene ID8350;8351;8352;8353;8354;8355;8356;8357;8358;8968;
• SDS-PAGE MW 17KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C/1 year

• Predicted MW: 17kd

  Western Blot: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

• Western blot analysis of extracts from C6 and Hela cells using Histone H3 (Di-Methyl-Lys27) Antibody #11583.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Histone H3 (Di-Methyl-Lys27) Antibody #11583.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting