• Product NameV5-Tag Mouse Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• IsotypeIgG1
• PurificationAffinity-chromatography
• ApplicationsWB IHC IF IP
• SpecificityV5-tag mouse monoclonal antibody detects endogenous levels of internal, C-terminal, and N-terminal V5- tagged proteins.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionA synthetic peptide GKPIPNPLLGLDST coupled to KLH.
• Target NameV5-Tag
• SDS-PAGE MW N/A
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20 °C.Stable for 12 months from date of receipt

• Western blotting      1:3000-1:10000
  Immunohistochemistry  1:200

Background: The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).
 

• All lanes : V5-tag mouse monoclonal antibody A: HEK 293 cell lysate transfected without V5-tag protein B: HEK 293 cell lysate transfected with V5 -tag protein C: IP (HEK 293 cell without transfected + anti- V5 mAb 4℃ overnight) D: IP (PBS + anti- V5 mAb 4℃ overnight) E: IP (HEK293 transfected+protein G+anti- V5 mAb 4℃ overnight) F: IP (HEK293 transfected+RIPA+Protein G 4℃ overnight) G: Multi Tag Recombinant protein (expressed in E.coli) TP=Target Protein HC=Heavy chain LC=Light Chain
• IF analysis of 293 cells transfected with a V5-tag protein, using anti-V5Tag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-V5)
• IP antibody use：5ug V5 Mouse IgG1 per ml Lysate，WB 1:5000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with V5-tag fusion protein 3、IP(untransfted 293+anti-V5 mAb+Protein G agarose) 4、IP (transfected 293+ normal Mouse IgG+Protein G agarose) 5、IP (transfected 293+anti-V5 mAb+ Protein G agarose) 6、IP (transfected 293+Protein G) 7、Recombinant protein (E.coli)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting