• Product NameBCA Protein Assay Kit

1.Storage the Protease Inhibitor Cocktail at -20?
2.Storage the extraction solution at 2-8?

BCA.2 Studies with di-, tri- and tetrapeptides suggest that the extent of color formation caused by more than the mere sum of individual color-producing functional groups.2 Accordingly, protein concentrations generally are determined and reported with reference to standards of a common protein such as bovine serum albumin (BSA). A series of dilutions of known concentration are prepared from the protein and assayed alongside the unknown(s) before the concentration of each unknown is determined based on the standard curve. If precise quantitation of an unknown protein is required, it is advisable to select a protein
standard that is similar in quality to the unknown; for example, a bovine gamma globulin (BGG) standard (see Related Thermo Scientific Products) may be used when assaying immunoglobulin samples.
Two assay procedures are presented. Of these, the Test Tube Procedure requires a larger volume (0.1mL) of protein sample; however, because it uses a sample to working reagent ratio of 1:20 (v/v), the effect of interfering substances is minimized. The Microplate Procedure affords the sample handling ease of a microplate and requires a smaller volume (10-25μL) of protein sample; however, because the sample to working reagent ratio is 1:8 (v/v), it offers less flexibility in overcoming interfering substance concentrations and obtaining low levels of detection.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting