• Product Namep53(Ab-18) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total p53 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 16~20 (Q-E-T-F-S) derived from Human p53.
• Target Namep53
• Alternative Names    Tumor suppressor p53; Phosphoprotein p53; Antigen NY-CO-13
• Accession No. Swiss-Prot: P04637
  NCBI Protein: NP_000537.3
• UniprotP04637
• Gene ID7157;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 53kd
  Western blotting: 1:500~1:1000
  

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.

Lin T, et al. (2005) Nat Cell Biol; 7(2): 165-71.
Vega FM, et al. (2004) Mol Cell Biol; 24(23): 10366-80.
Li J, et al. (2004) J Biol Chem; 279(40): 41275-9.
Wang J, et al. (2004) J Biol Chem; 279(38): 39584-92.

• Western blot analysis of extracts from HT29 cells using p53(Ab-18) Antibody #21086.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting