• Product NameRecombinant Murine Noggin(rMuNoggin)
• Brief DescriptionRecombinant Protein
• Host SpeciesE.coli
• Purification> 95 % by SDS-PAGE and HPLC analyses.
• Species ReactivityMs
• Biological ActivityFully biologically active when compared to standard. The ED50 as determined by inhibiting BMP-4-induced alkaline phosphatase production of murine ATDC5 cells is less than 2 ng ml, corresponding to a specific activity of > 5.0 ⅹ 105 IU mg in the prese
• EndotoxinLess than 1 EU/��g of rMuNoggin as determined by LAL method.
• Target Namerm Noggin
• Accession No. accession:P97466
  GeneID:18121
• UniprotP97466
• Gene ID18121;
• Calculated MW Approximately 46.4 kDa, a disu
• SDS-PAGE MW Sterile Filtered White lyophil
• Target Sequence MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGPAG GAEDLAELDQ LLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVG SCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGGQRCG WIPIQYPIIS ECKCSC
• Formulation Lyophilized from a 0.2 ��m filtered concentrated solution in 30 % acetonitrile, 0.1 % TFA.
• Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HAc to a concentration less than 0.25 mg mL. Stock solutions should be apportioned into working aliquots and stored at �� -20 &#
• Storage This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.

Noggin encoded by the NOG gene, was first isolated from Xenopus, having the function of inducing secondary axis formation in frog embryos. It inhibits TGF-β family ligands and preventing them from binding to their corresponding receptors. Noggin was originally found as a BMP-4 antagonist, and then has been shown to modulate the activities of other BMPs (BMP-2, 7, 13 and 14). Additionally, it has pleiotropic effect, both in early development and later stages. The results of the mouse knockout of noggin suggest that it is involved in numerous developmental processes, such as neural tube fusion and joint formation. In recent report, proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) have relation with the mutant of evolutionarily conserved amino acid residues of Noggin. Mature mouse Noggin shares 99 % and 83 % a.a. sequence identity with human and Xenopus Noggin, respectively.

1. Davis SWandCamper SA. 2007. Dev Biol, 305: 145-60. 2. Zhu W, Kim J, Cheng C, et al. 2006. Bone, 39: 61-71. 3. Oxley CD, Rashid R, Goudie DR, et al. 2008. Horm Res, 69: 221-6. 4. Cooper GM, Usas A, Olshanski A, et al. 2009. Plast Reconstr Surg, 123: 94S-103S. 5. Bayramov AV, Eroshkin FM, Martynova NY, et al. 2011. Development, 138: 5345-56.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting