• Product NameElk1(Phospho-Thr417) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsIHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of Elk1 only when phosphorylated at threonine 417.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 417(L-S-T(p)-P-V) derived from Human Elk1.
• Target NameElk1
• ModificationPhospho
• Alternative Names    ELKV
• Accession No. Swiss-Prot: P19419
  NCBI Gene ID: 2002
  NCBI mRNA: NM_001257168.1
  NCBI Protein: NP_001244097.1
• UniprotP19419
• Gene ID2002;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C

• Predicted MW: 47kd

  Immunohistochemistry: 1:50~1:100

  Immunofluorescence: 1:100~1:200

 Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.

Yang S.-H., Sharrocks A.D.EMBO J. 24:2161-2171(2005)

Zhang L., Yang S.H., Sharrocks A.D.Mol. Cell. Biol. 27:2861-2869(2007) 

• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Elk-1 (phospho-Thr417) antibody (#11038).
• Immunofluorescence staining of methanol-fixed HeLa cells using Elk-1 (phospho-Thr417) antibody (#11038, Red).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting