• Product NameBAD(Phospho-Ser155) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of BAD only when phosphorylated at serine 155.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 155(R-M-S(p)-D-E) derived from Human BAD.
• Target NameBAD
• ModificationPhospho
• Alternative Names    Bbc2; AI325008
• Accession No. Swiss-Prot: Q61337
  NCBI Gene ID: 12015
  NCBI mRNA: NM_007522.2
  NCBI Protein: NP_031548.1
• UniprotQ61337
• Gene ID12015;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C

• Predicted MW: 23kd

  Western blotting: 1:500

  Immunohistochemistry: 1:50~1:100

 Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

• Western blot analysis of extracts from 293 cells using BAD (phospho-Ser155) antibody (#11069).
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD (phospho- Ser155) antibody (#11069).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting