• Product NameERK1 2(Phospho-Thr202 + Tyr204) Antibody HRP Conjugated
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationPurified by Protein A.
• ApplicationsWB  IHC-P IHC-F ICC
• Species ReactivityHu Ms Rt   
• Immunogen DescriptionKLH conjugated synthetic phosphopeptide aa 196-210 379 derived from human p44 42 MAPK around the phosphorylation site of (Thr202 Tyr204)
• ConjugatesHRP
• Excitation EmissionN A
• Target NameERK1 2 Thr202 + Tyr204
• Alternative Names    ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK; Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; Extracellular signal-regulated kinase 1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; Microtubule-associated protein 2 kinase; MAPK3
• Accession No. Swiss-Prot#P27361
  NCBI Gene ID5595
• UniprotP27361
• Gene ID5595;
• Cell Localization Cytoplasm, Nucleus
• Concentration 1mg ml
• Formulation 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• Storage Shipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.

• WB=1:500-2000  IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200

p44 42 MAP Kinase(Thr202); ERK (extracellular signal regulated kinase), also known as MAPK (mitogen activated protein kinase) has two closely related isoforms of 44 kDa and 42 kDa, respectively. These kinases belong to a family of serine threonine kinases that are activated upon treatment of cells with a large variety of stimuli including mitogens, hormones, growth factors, cytokines, and bioactive peptides. Cell stimulation induces the activation of a signaling cascade, the downstream effects of which have been linked to the regulation of cell growth and differentiation as well as the cytoskeleton. ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK ERK kinases), thereby greatly elevating the activity of ERK1&2.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting