• Product NameANGPTL3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total ANGPTL3 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to C terminal 300 amino acids of Human Angiopoietin-related protein 3
• Target NameANGPTL3
• Alternative Names    Angiopoietin-related protein 3, FHBL2, ANGPT5
• Accession No. Swiss-Prot:Q9Y5C1
  Gene ID:27329;
• UniprotQ9Y5C1
• Gene ID27329;
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 54kd
  ELISA: 1:1000-1:5000
  Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is predominantly expressed in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain and the C-terminal fibrinogen (FBN)-like domain. The FBN-like domain in angiopoietin-like 3 protein was shown to bind alpha-5/beta-3 integrins, and this binding induced endothelial cell adhesion and migration. This protein may also play a role in the regulation of angiogenesis.

• Gel: 10%SDS-PAGE
  Lysate: 30 µg Human liver cancer tissue lysate
  Primary antibody: 1/400 dilution
  Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/ 3000 dilution
  Exposure time: 1 minute
• The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 31029(ANGPTL3 Antibody) at dilution 1/8, on the right is treated with the fusion protein.
  
  

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting