• Product NameIRS1(Phospho-Tyr612) Antibody HRP Conjugated
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationPurified by Protein A.
• ApplicationsWB  IHC-P IHC-F
• Species ReactivityHu Ms Rt   
• Immunogen DescriptionKLH conjugated synthetic phosphopeptide aa 580-630 1242 derived from human IRS1 around the phosphorylation site of Tyr612 [DG(p-Y)MP]
• ConjugatesHRP
• Excitation EmissionN A
• Target NameIRS1 Tyr612
• Alternative Names    HIRS-1; Insulin receptor substrate 1; IRS-1; IRS1
• Accession No. Swiss-Prot#P35568
  NCBI Gene ID3667
• UniprotP35568
• Gene ID3667;
• Cell Localization Cytoplasm, Nucleus
• Concentration 1mg ml
• Formulation 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• Storage Shipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.

• WB=1:500-2000  IHC-P=1:50-200 IHC-F=1:50-200 

May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting