• Product NameCBX8 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total CBX8 protein.
• Immunogen TypeRecombinant protein
• Immunogen DescriptionFusion protein corresponding to a C terminal 300 amino acids of human chromobox homolog 8
• Target NameCBX8
• Alternative Names    chromobox homolog 8, PC3, RC1
• Accession No. Swiss-Prot:Q9HC52
  Gene ID:57332;
• UniprotQ9HC52
• Gene ID57332;
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 43kd
  ELISA: 1:2000-1:5000
  Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:10-1:50

Chromobox protein homolog 8 is a protein that in humans is encoded by the CBX8 gene.CBX8 has been shown to interact with RING1 and MLLT1.Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.

• Gel: 10%SDS-PAGE
  Lane1: 231 cell lysate
  Lane2: Hela cell lysate
  Lane3: A549 cell lysate
  Lysates: 40 ug
  Primary antibody: 1/200 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
  Exposure time: 1 minute
  
• The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 31048 (CBX8 Antibody) at dilution 1/10, on the right is treated with the fusion protein.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting