• Product NameCaveolin-1(Ab-14) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total Caveolin-1 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.12~16 (H-L-Y-T-V) derived from Human CAVEOLIN-1.
• Target NameCaveolin-1
• Alternative Names    CAV; CAV1;
• Accession No. Swiss-Prot: Q03135
  NCBI Protein: NP_001166366.1
• UniprotQ03135
• Gene ID857;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 24kd
  Western blotting: 1:500~1:1000
  

The scaffolding protein encoded by Caveolin-1 is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (a and beta) are encoded by a single transcript from this gene.

Zhang Y, et al. (2005) Mol Cell Proteomics. 4(9): 1240-1250.
Labrecque L, et al. (2004) J Biol Chem. 279(50): 52132-52140.
Fielding PE, et al. (2004) Biochemistry. 43(9): 2578-2586.
Labrecque L, et al. (2003) Mol Biol Cell. 14(1): 334-347.
Maggi D, et al. (2002) Biochem Biophys Res Commun. 295(5): 1085-1089.

• Western blot analysis of extracts from HT29 and HUVEC cells using Caveolin-1(Ab-14) Antibody #21112.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting