• Product NameGSR Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total GSR protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to a region derived from 274-475 amino acids of human glutathione reductase
• Target NameGSR
• Alternative Names    Glutathione reductase
• Accession No. Swiss-Prot:P00390
  Gene ID:2936;
• UniprotP00390
• Gene ID2936;
• Concentration 0.4mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 56kd
  ELISA: 1:2000-1:10000
  Western blotting: 1:1000-1:5000
  Immunohistochemistry: 1:50-1:200

This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found.

• Gel: 10%SDS-PAGE
  Lane1: Jurkat cell lysate
  Lane2: Hela cell lysate
  Lane3: A549 cell lysate
  Lysates: 40 ug per lane
  Primary antibody: 1/750 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
  Exposure time: 30 seconds
  
• The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using 31078 (GSR Antibody) at dilution 1/50, on the right is treated with the fusion protein.
  
  

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting