• Product NameTRAP1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total TRAP1 protein.
• Immunogen TypeRecombinant protein
• Immunogen DescriptionFusion protein corresponding to a C terminal 300 amino acids of human TNF receptor-associated protein 1
• Target NameTRAP1
• Alternative Names    TNF receptor-associated protein 1, HSP75, HSP90L
• Accession No. Swiss-Prot:Q12931
  Gene ID:10131;
• UniprotQ12931
• Gene ID10131;
• Concentration 0.6mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 80kd
  ELISA: 1:2000-1:10000
  Western blotting: 1:1000-1:5000
  Immunohistochemistry: 1:100-1:200

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. TRAP1 is a mitochondrial HSP90 protein. Other HSP90 proteins are found in cytosol and endoplasmic reticulum

• Gel: 10%SDS-PAGE
  Lane1: K562 cell lysate
  Lane2: Jurkat cell lysate
  Lane3: 293T cell lysate
  Lysates: 30ug per lane
  Primary antibody: 1/1050 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
  Exposure time: 1 minute
  
• The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 31085 (TRAP1 Antibody) at dilution 1/60, on the right is treated with the fusion protein.
  
  
  

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting