• Product NameIL18 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total IL18 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFull length fusion protein
• Target NameIL18
• Alternative Names    interleukin 18 , IGIF, IL-18, IL-1g, IL1F4
• Accession No. Swiss-Prot:Q14116
  Gene ID:3606;
• UniprotQ14116
• Gene ID3606;
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 22kd
  ELISA: 1:2000-1:5000
  Western blotting: 1:1000-1:2000
  Immunohistochemistry: 1:100-1:200

The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene

• Gel: 12%SDS-PAGE
  Lysate: 30 µg Hela cell lysate
  Primary antibody: 1/1350 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
  Exposure time: 5 seconds
• The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 31089 (IL18 Antibody) at dilution 1/100, on the right is treated with the fusion protein.
  
  
  

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting