• Product NameSmad3 (Phospho-Ser423 + Ser425) Antibody FITC Conjugated
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationPurified by Protein A.
• ApplicationsFlow-Cyt ICC IF
• Species ReactivityHu Ms Rt   
• Immunogen DescriptionKLH conjugated synthetic phosphopeptide aa 380-425 425 derived from human Smad3 around the phosphorylation site of Ser423 425 [CS(p-S)V(p-S)]
• ConjugatesFITC
• Excitation Emission494nm 518nm
• Target NameSmad3 Ser423 + Ser425
• Alternative Names    LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD family member 3; SMAD 3; Smad3; hSMAD3
• Accession No. Swiss-Prot#P84022
  NCBI Gene ID4088
• UniprotP84022
• Gene ID4088;
• Cell Localization Cytoplasm, Nucleus
• Concentration 1mg ml
• Formulation 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• Storage Shipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.

• Flow-Cyt=1:50-200 ICC=1:50-200 IF=1:50-200

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3 SMAD4 complex, activates transcription. Also can form a SMAD3 SMAD4 JUN FOS complex at the AP-1 SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting