• Product NameIkB-a(Ab-32/36) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of total IkB-a protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.30~34/34~38 (H-D-S-G-L- D-S -M-K) derived from Human IkB-a.
• Target NameIkB-a
• Alternative Names    I-kappa-B-alpha; IKBA; NF-kappaB inhibitor alpha; NFKBI; NFKBIA
• Accession No. Swiss-Prot: P25963
  NCBI Protein: NP_065390.1
• UniprotP25963
• Gene ID4792;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 39kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.

Mattioli I, et al. (2004)J Immunol; 172(10): 6336-44.
Courtois G, et al. (2003)J Clin Invest; 112(7): 1108-15.
Nair A, et al. (2003) Oncogene; 22(1): 50-8.
Fan C, et al. (2002)J Cell Sci; 115(Pt 24): 4843-53.
Schubert SY, et al. (2002)FASEB J; 16(14): 1931-3.

• Western blot analysis of extracts from 293 and 3T3 cells using IkB-a(Ab-32/36) Antibody #21122.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-a(Ab-32/36) Antibody #21122(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed Hela cells using IkB-a(Ab-32/36) Antibody #21122.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting