• Product NamePFN1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total PFN1 protein.
• Immunogen TypeRecombinant protein
• Immunogen DescriptionFusion protein corresponding to a region derived from 2-140 amino acids of human profilin 1
• Target NamePFN1
• Alternative Names    Profilin 1, ALS18
• Accession No. Swiss-Prot:P07737
  Gene ID:5216;
• UniprotP07737
• Gene ID5216;
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 15kd
  ELISA: 1:1000-1:2000
  Western blotting: 1:200-1:1000

This gene encodes a member of the profilin family of small actin-binding proteins. The encoded protein plays an important role in actin dynamics by regulating actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome, and the encoded protein may also play a role in Huntington disease. Multiple pseudogenes of this gene are located on chromosome 1.?

• Gel: 12%SDS-PAGE
  Lane1: Hela cell lysate
  Lane2: HT-29 cell lysate
  Lane3: A549 cell lysate
  Lysates: 40 ug per lane
  Primary antibody: 1/200 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
  Exposure time: 10 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting