• Product NameASK1(Ab-83) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total ASK1 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.81~85 (G-S-S-V-G) derived from Human ASK1.
• Target NameASK1
• Alternative Names    ASK-1; M3K5; MAP3K5; MAPK/ERK kinase kinase 5; MAPKKK5
• Accession No. Swiss-Prot: Q99683
  NCBI Protein: NP_005914.1
• UniprotQ99683
• Gene ID4217;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 155kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.

Mabuchi S, et al. (2004) Endocrinology. 145(1): 49-58.
Yuan ZQ, et al. (2003) J Biol Chem. 278(26): 23432-23440.
Kim AH, et al. (2001) Mol Cell Biol. 21(3): 893-901.

• Western blot analysis of extracts from MDA-MB- 435 cells using ASK1(Ab-83) Antibody #21125 and the same antibody preincubated with blocking peptide.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ASK1(Ab-83) Antibody #21125(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed Hela cells using ASK1(Ab-83) Antibody #21125.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting