• Product NameeNOS (Phospho-Thr495) Antibody FITC Conjugated
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationPurified by Protein A.
• ApplicationsIF
• Species ReactivityHu Ms Rt
• Immunogen DescriptionKLH conjugated synthetic phosphopeptide aa 470-520 1203 derived from human eNOS around the phosphorylation site of Thr495
• ConjugatesFITC
• Excitation Emission494nm 518nm
• Target NameeNOS Thr495
• Alternative Names    eNOS; ECNOS; Nitric oxide synthase, endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; NOS type III; NOSIII; NOS3
• Accession No. Swiss-Prot#P29474
  NCBI Gene ID4846
• UniprotP29474
• Gene ID4846;
• Cell Localization Cytoplasm, Cell membrane
• Concentration 1mg ml
• Formulation 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• Storage Shipped at 4˚C. Store at -20˚C for one year. Avoid repeated freeze/thaw cycles.

• IF=1:50-200

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting