• Product NamePYGB Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total PYGB protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from 1-15 amino acids of Human phosphorylase, glycogen; brain
• Target NamePYGB
• Alternative Names    Phosphorylase, glycogen; brain, GPBB
• Accession No. Swiss-Prot:P11216
  Gene ID:5834;
• UniprotP11216
• Gene ID5834;
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 97kd
  ELISA: 1:1000-1:5000
  Western blotting: 1:500-1:2000

The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation

• Gel: 8%SDS-PAGE
  Lysate: 40 µg HT-29 cell lysate
  Primary antibody: 1/550 dilution
  Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
  Exposure time: 10 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting