• Product NameADAMTS5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsELISA WB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of total ADAMTS5 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from 529-541 amino acids of Human A disintegrin and metalloproteinase with thrombospondin motifs 5
• Target NameADAMTS5
• Alternative Names    A disintegrin and metalloproteinase with thrombospondin motifs 5, ADMP-2, ADAM-TS5, ADAMTS-5, ADAMTS11, ADAM-TS 5, ADAMTS-11, ADAM-TS 11
• UniprotQ9UNA0
• Concentration 1.1 mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C/1 year

• Predicted MW: 102kd
  ELISA: 1:1000-1:5000
  Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:10-1:50

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene contains two C-terminal TS motifs and functions as aggrecanase to cleave aggrecan, a major proteoglycan of cartilage.

• Gel: 6%SDS-PAGELysate: 40 μgLane: K562 cell lysatePrimary antibody: ADAMTS5 Antibody at dilution 1/200Secondary antibody: Goat anti rabbit IgG at 1/5000 dilutionExposure time: 3 minutes
• The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ADAMTS5 Antibody at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting